Product Information Sheet

Catalog #:E02030
Description:10X coating buffer. pH 9.5
Storage:Short term store at 2-8 oC to 6 months).
Instruction:Make 10-fold dilution of the 10X assay diluent buffer (1 part buffer with 9 part of distilled or de-ionized water). Working solution (after dilution) can be stored at room temperature for up to 2 weeks.
Notes:Higher pH normally induces some conformational changes of the antibody or antigen to allow more exposure of the hydrophobic regions of the protein, thus facilitating the non-specific binding to the solid surface.
Application:This assay diluent can be used in alkaline phosphatase-based ELISA or blotting.

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